Protein profiles of dengue-infected Aedes aegypti (L)
ResumenThe protein profiles of DENV-2 and DENV-4 viruses-infected adult Aedes aegypti were analysed using Coomassie-stained SDS-PAGE samples. For this study, adult female mosquitoes were infected orally with either DENV-2 or DENV-4 virus using spiked human blood samples. The infections in the individual mosquitoes were confirmed using the reverse transcription-polymerase chain reaction (RT-PCR). From the SDS-PAGE analyses, a total of 11 proteins with molecular weights of 22 kDa to greater than 181.8 kDa were identified in DENV-2 and DENV-4-infected mosquito homogenates. Three proteins (Mr 49, 64 and 200 kDa) from DENV-2-infected mosquitoes and five proteins (Mr 49, 50, 60, 135 and 200 kDa) from DENV-4-infected mosquitoes were identified at higher concentrations than in uninfected controls. Further work is now needed to specifically identify these proteins and study their biological roles in DENV infections.
Lee, H.L., Wong, Y C & Rohan, A i. (2009). Protein profiles of dengue-infected Aedes aegypti (L). WHO Regional Office for South-East Asia.. http://www.who.int/iris/handle/10665/170926