• The Interdomain Region of Dengue NS5 Protein Interacts with NS3 and Host Proteins. 

      G Vasudevan, Subhash; Jans, David A; Criswell, Erin; Johansson, Magnus; Brooks, Andrew J (‏2002-12)‏
    • The interdomain region of dengue NS5 protein interacts with NS3 and host proteins. 

      Vasudevan, Subhash G; Jans, David A; Criswell, Erin; Johansson, Magnus; Brooks, Andrew J (‏2002)‏
      Although dengue virus genome replication occurs in the cytoplasm of infected cells, it has been shown that the NS5 protein (‏RNA-dependent RNA polymerase)‏ is hyperphosphorylated at a late stage in infection and localized to the cell nucleus. A 37 amino acid sequence of NS5 (‏residues 369-405)‏was shown to contain a functional unclear localization signal (‏NLS)‏ that interacted with the cellular nuclear transport factor, importin α/β heterodimer. Further studies using the yeast two-hybrid system revealed that the NS5 region (‏residues 320-368)‏ immediately ...