Effect of water softening on the colour intensity of routine haematoxylin and eosin stain
AbstractHaematoxylin and eosin [H and E] is the most popular routine stain used in pathology laboratories for highlighting cellular structures. To study the effect of tap water'softening' [i.e. calcium extraction] on H and E stains, 5 sets of slides from 30 different paraffin-embedded human pathologic tissue blocks were prepared in the same way except for washing with 5 different types of water. Slides washed in untreated tap water showed the best results concerning differentiation and colour intensity, while slides washed with softened or other treated water showed poorer degrees of differentiation and colour intensity. The worst results were obtained from slides washed with water containing sodium bicarbonate. Low calcium and magnesium ions and high sodium ions in soft water adversely affect the results of routine H and E stain
Malkawi, S.R., Abu Hazeem, R.M. & Easa, M.O. (2003). Effect of water softening on the colour intensity of routine haematoxylin and eosin stain. EMHJ - Eastern Mediterranean Health Journal, 9 (5-6), 1109-1113, 2003 https://apps.who.int/iris/handle/10665/119367
JournalEMHJ - Eastern Mediterranean Health Journal, 9 (5-6), 1109-1113, 2003
MetadataShow full item record
Showing items related by title and subject
Khoury, S.; Graczyk, T.; Burnham, G.; Jurdi, M.; Goldman, L. (World Health Organization. Regional Office for the Eastern Mediterranean, 2016-08)Drinking water at Shatila Palestinian Refugee Camp in Beirut, Lebanon is of poor quality and unpredictably intermittent quantity. We aimed to characterize drinking water sources and contamination at Shatila and determine ...
Workshop on Household Water Treatment and Safe Storage (HWTS), Siem Reap, Cambodia, 10-12 October 2006 : report World Health Organization. Regional Office for the Western Pacific (Manila : WHO Regional Office for the Western Pacific, 2006)