Evaluation of 2-column agglutination versus conventional tube technique for antibody screening
AbstractThe study aimed to determine the specificity and sensitivity of the Ortho BioVue two-column agglutination system for the detection of low concentrations of clinically significant antibodies in serum. The BioVue system was compared with the conventional tube technique [LISS-Coombs indirect antiglobulin test], and the two-stage Papenzyme test was used to resolve discrepancies between the two methods. We tested 3000 serum samples from randomly selected patients at King Hussein Medical Centre. Both the antibody screening and identification gave negative results in 2952 patients and positive results in 48 patients. We found the BioVue system to be the more sensitive technique. However, if papain enzyme-treated cells were included in the conventional tube technique when applied to antibody screening and identification, both methods would be of comparable sensitivity
Abou Jabal, A., Shubeilat, T. & Hajjiri, F. (2003). Evaluation of 2-column agglutination versus conventional tube technique for antibody screening. EMHJ - Eastern Mediterranean Health Journal, 9 (3), 407-412, 2003 http://www.who.int/iris/handle/10665/119291
JournalEMHJ - Eastern Mediterranean Health Journal, 9 (3), 407-412, 2003
MetadataShow full item record
Showing items related by title and MeSH subject.
Automated agglutination reactions for detection of Australia antigen, t. cruzi antipolysaccharide anti-bodies, and anti-salmonella flagellar antibodies Pereira, Carlos Augusto; Mimica Mimica, Igor; Kiyomi Takeda, Augusta (1978)
Variation on antibody titres determined by the indirect fluorescent antibody test using homologous antigen in persons infected with the chesson strain of Plasmodium vivax / by A. J. Lysenko ... [et al.] Lysenko, A. J; Glazunova, Z. I; Nikitin, V. A; Doroshenko, M. I; World Health Organization (WHO/MAL/77.889, 1977)