Primary isolation and detection of Helicobacter pylori from dyspeptic patients: a simple, rapid method
AbstractThe study aimed to develop a rapid and simple method for the primary isolation and detection of Helicobacter pylori from dyspeptic patients. Mucosal antral biopsy specimens were obtained from 136 consecutive dyspeptic patients diagnosed with peptic ulcer by endoscopy at Basra General Hospital, Iraq. From histopathological examination of biopsies, H. pylori was detected in 81 [59.6%] peptic ulcer patients. For bacterial culture, specimens were cultured in parallel on 2 media: the nonselective classic Columbia agar and the selective modified Columbia urea agar [MCUA]. MCUA showed a higher isolation rate than classic Columbia agar [67.6% versus 44.1% of patients], and the results were obtained faster [24 hours versus 5-7 days] with more clear-cut identification
Al Sulami, A., Al Kiat, H.S., Bakker, L.K. & Hunoon, H. (2008). Primary isolation and detection of Helicobacter pylori from dyspeptic patients: a simple, rapid method. http://www.who.int/iris/handle/10665/117434
EMHJ - Eastern Mediterranean Health Journal, 14 (2), 268-276, 2008
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Alimohammadi, H.; Fouladi, N.; Salehzadeh, F.; Alipour, S.A.; Javadi, M.S. (2016-12)We examined the role of Helicobacter pylori infection as a cause of recurrent abdominal pain [RAP] among Iranian children in a population-based case-control study to determine the association between H. pylori infection and RAP among schoolchildren. A total of 1558 children aged 6-13 years were examined. Children with RAP confirmed by the Apley and Naish criteria were selected; 145 cases were selected for inclusion and were compared with 145 healthy children recruited from the same area. Both groups underwent stool antigen testing. The prevalence ...
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