Anabolic Effects of Whey and Casein After Strength Training in Young and Elderly

• Register: ClinicalTrials.gov
• Last refreshed on: 12 December 2020
• Main ID: NCT02968888
• Date of registration: 10/11/2016
• Prospective Registration: No
• Primary sponsor: Norwegian School of Sport Sciences
• Public title: Anabolic Effects of Whey and Casein After Strength Training in Young and Elderly
• Scientific title: Effects of Whey and Casein Supplementation on Acute Anabolic Responses in Muscle After Strength Training in Young and Elderly
• Date of first enrolment: August 2014
• Target sample size: 43
• Recruitment status: Completed
• URL: https://clinicaltrials.gov/show/NCT02968888
• Study type: Interventional
• Study design: Allocation: Randomized. Intervention model: Crossover Assignment. Primary purpose: Basic Science. Masking: Triple (Participant, Care Provider, Outcomes Assessor).
• Phase: N/A

Countries of recruitment

Norway

Key inclusion & exclusion criteria

Inclusion Criteria:

- Healthy in the sense that they can conduct training and testing

- Able to understand Norwegian language written and oral

- Between 18 and 45, or above 70 years of age

Exclusion Criteria:

- Diseases or injuries contraindicating participation

- Use of dietary supplements (e.g. proteins, vitamins and creatine)

- Lactose intolerance

- Allergy to milk

- Allergy towards local anesthetics (xylocain)



Age minimum: 18 Years
Age maximum: N/A
Gender: All

Health Condition(s) or Problem(s) studied

Elderly

Healthy

Young

Intervention(s)

Other: Strength training

Dietary Supplement: Native whey

Dietary Supplement: Milk 1%

Dietary Supplement: Whey protein concentrate 80

Primary Outcome(s)

Mixed muscle fractional synthetic rate [Time Frame: One to five hours after a bout of strength training and protein consumption]

Mixed muscle fractional breakdown rate [Time Frame: From three to five hours after a bout of strength training and protein consumption]

Mixed muscle fractional synthetic rate [Time Frame: Three to one hours prior to a bout of strength training and protein consumption]

Mixed muscle fractional synthetic rate [Time Frame: From three to five hours after a bout of strength training and protein consumption]

Secondary Outcome(s)

Change in C-C motif chemokine 3 (CCL3) mRNA [Time Frame: 1 hour after training and protein intake]

Change in C-C motif chemokine 5 (CCL5) mRNA [Time Frame: 1 hour after training and protein intake]

Change in ABCA1 mRNA [Time Frame: 5 hous after training and protein intake]

Change in ABRA1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in CD36 mRNA [Time Frame: 5 hours after training and protein intake]

Change in cholesterol 25-hydroxylase (CH25H) mRNA [Time Frame: 1 hour after training and protein intake]

Change in ATP-binding cassette transporter (ABCA1) messenger ribonucleic acid (mRNA) [Time Frame: 1 hour after training and protein intake]

Change in IL10 mRNA [Time Frame: 5 hours after training and protein intake]

Change in IL8 mRNA [Time Frame: 5 hours after training and protein intake]

Change in C-C motif chemokine 2 (CCL2) mRNA [Time Frame: 1 hour after training and protein intake]

Change in E3 ubiquitin-protein ligase TRIM63 (TRIM63) mRNA [Time Frame: 1 hour after training and protein intake]

Change in interleukin-1 receptor antagonist protein (IL1RN) mRNA [Time Frame: 1 hour after training and protein intake]

Change in IGF1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in JUNB mRNA [Time Frame: 5 hours after training and protein intake]

Change in large neutral amino acids transporter small subunit 1 (SLC7A5) mRNA [Time Frame: 1 hour after training and protein intake]

Change in 4F2 cell-surface antigen heavy chain (SLC3A2) mRNA [Time Frame: 1 hour after training and protein intake]

Change in ACTA1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in KITLG mRNA [Time Frame: 5 hours after training and protein intake]

Change in myostatin (MSTN) mRNA [Time Frame: 1 hour after training and protein intake]

Change in matrix metalloproteinase-9 (MMP9) mRNA [Time Frame: 1 hour after training and protein intake]

Change in myosin-7 (MYH7) mRNA [Time Frame: 1 hour after training and protein intake]

Change in CCL2 mRNA [Time Frame: 5 hours after training and protein intake]

Change in forkhead box protein O1 (FOXO1) mRNA [Time Frame: 1 hour after training and protein intake]

Change in HGF mRNA [Time Frame: 5 hours after training and protein intake]

Change in IL1B mRNA [Time Frame: 5 hours after training and protein intake]

Change in C-X-C motif chemokine 16 (CXCL16) mRNA [Time Frame: 1 hour after training and protein intake]

Change in CXCL1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in IL6 mRNA [Time Frame: 5 hours after training and protein intake]

Change in interleukin-1B (IL1B) mRNA [Time Frame: 1 hour after training and protein intake]

Change in granulocyte colony-stimulating factor (CSF3) mRNA [Time Frame: 1 hour after training and protein intake]

Serum ureic acid [Time Frame: 180 and 60 min before, and 60, 10, 180 and 300 min after training and protein intake]

Change in FBXO32 mRNA [Time Frame: 5 hours after training and protein intake]

Change in hepatocyte growth factor (HGF) mRNA [Time Frame: 1 hour after training and protein intake]

Change in insulin-like growth factor I (IGF1) mRNA [Time Frame: 1 hour after training and protein intake]

Change in myosin-1 (MYH1) mRNA [Time Frame: 1 hour after training and protein intake]

Ratio of phosphorylated to total ribosomal protein S6 kinase beta-1(P70S6K) change from baseline [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Change in PPARGC1A mRNA [Time Frame: 5 hours after training and protein intake]

Change in prostaglandin G/H synthase 2 (PTGS2) mRNA [Time Frame: 1 hour after training and protein intake]

Change in proton-coupled amino acid transporter 1 (SLC36A1) mRNA [Time Frame: 1 hour after training and protein intake]

Intracellular translocation of muscle RING-finger protein-1 (Murf-1) change from baseline [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Plasma insulin [Time Frame: 180 and 60 min before, and 45, 60, 75, 120, 160, 180, 200, 220 and 300 min after training and protein intake]

Serum urea [Time Frame: 180 and 60 min before, and 60, 10, 180 and 300 min after training and protein intake]

Change in CCL3 mRNA [Time Frame: 5 hours after training and protein intake]

Change in CCL5 mRNA [Time Frame: 5 hours after training and protein intake]

Change in growth-regulated alpha protein (CXCL1) mRNA [Time Frame: 1 hour after training and protein intake]

Change in IL17D mRNA [Time Frame: 5 hours after training and protein intake]

Change in interleukin-17D (IL17D) mRNA [Time Frame: 1 hour after training and protein intake]

Change in kit ligand (KITLG) mRNA [Time Frame: 1 hour after training and protein intake]

Change in MSTN mRNA [Time Frame: 5 hours after training and protein intake]

Change in MYH1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in MYH2 mRNA [Time Frame: 5 hours after training and protein intake]

Change in myosin-2 (MYH2) mRNA [Time Frame: 1 hour after training and protein intake]

Change in SLC7A5 mRNA [Time Frame: 5 hours after training and protein intake]

Change in TNF mRNA [Time Frame: 5 hours after training and protein intake]

Change in transcription factor jun-B (JUNB) mRNA [Time Frame: 1 hour after training and protein intake]

Plasma amino acid concentration [Time Frame: 180 and 60 min before, and 45, 60, 75, 120, 160, 180, 200, 220 and 300 min after training and protein intake]

Change in oxysterols receptor LXR-alpha (NR1H3) mRNA [Time Frame: 1 hour after training and protein intake]

Change in peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) mRNA [Time Frame: 1 hour after training and protein intake]

Change in SLC36A1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in SLC3A2 mRNA [Time Frame: 5 hours after training and protein intake]

Change in alfa-actin (ACTA1) mRNA [Time Frame: 1 hour after training and protein intake]

Change in C-C motif chemokine 8 (CCL8) mRNA [Time Frame: 1 hour after training and protein intake]

Change in sodium-coupled neutral amino acid transporter 2 (SLC38A2) mRNA [Time Frame: 1 hour after training and protein intake]

Change in CCL8 mRNA [Time Frame: 5 hours after training and protein intake]

Change in CXCL16 mRNA [Time Frame: 5 hours after training and protein intake]

Muscle force generating capacity change from baseline [Time Frame: 15 min before, 15 and 300 min after, and 24 hours after training and protein intake]

Change in E3 ubiquitin-protein ligase TRIM63 (TRIM63) mRNA [Time Frame: 5 hours after training and protein intake]

Change in F-box only protein 32 (FBXO32) mRNA [Time Frame: 1 hour after training and protein intake]

Change in BRCA1-A complex subunit Abraxas (ABRA1) mRNA [Time Frame: 1 hour after training and protein intake]

Change in CH25H mRNA [Time Frame: 5 hours after training and protein intake]

Change in CSF3 mRNA [Time Frame: 5 hours after training and protein intake]

Change in forkhead box protein O3 (FOXO3A) mRNA [Time Frame: 1 hour after training and protein intake]

Change in FOXO1 mRNA [Time Frame: 5 hours after training and protein intake]

Change in IL1RN mRNA [Time Frame: 5 hours after training and protein intake]

Change in interleukin-10 (IL10) mRNA [Time Frame: 1 hour after training and protein intake]

Change in MMP9 mRNA [Time Frame: 5 hours after training and protein intake]

Change in NR1H3 mRNA [Time Frame: 5 hours after training and protein intake]

Change in nuclear receptor subfamily 4 group A member 3 (NR4A3) mRNA [Time Frame: 1 hour after training and protein intake]

Change in NR4A3 mRNA [Time Frame: 5 hours after training and protein intake]

Change in platelet glycoprotein 4 (CD36) mRNA [Time Frame: 1 hour after training and protein intake]

Change in FOXO3A mRNA [Time Frame: 5 hours after training and protein intake]

Change in interleukin-6 (IL6) mRNA [Time Frame: 1 hour after training and protein intake]

Plasma glucose [Time Frame: 180 and 60 min before, and 45, 60, 75, 120, 160, 180, 200, 220 and 300 min after training and protein intake]

Change in interleukin-8 (IL8) mRNA [Time Frame: 1 hour after training and protein intake]

Change in MYH7 mRNA [Time Frame: 5 hours after training and protein intake]

Change in PTGS2 mRNA [Time Frame: 5 hours after training and protein intake]

Phosphorylation of phosphorylated to total eukaryotic elongation factor 2 (eEF-2) change from baseline [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Change in SLC38A2 mRNA [Time Frame: 5 hours after training and protein intake]

Intracellular translocation of forkhead box O3 (FOXO3a) change from baseline [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Change in TLR2 mRNA [Time Frame: 5 hours after training and protein intake]

Change in toll-like receptor 2 (TLR2) mRNA [Time Frame: 1 hour after training and protein intake]

Change in tumor necrosis factor (TNF) mRNA [Time Frame: 1 hour after training and protein intake]

Intracellular translocation of Atrogin1 change from baseline [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Phosphorylation of phosphorylated to total eukaryotic translation initiation factor 4E-binding protein 1 (4EBP-1) change from baseline [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Ubiquitin [Time Frame: 30 min before, 1, 2.5 and 5 hours after training and protein intake]

Serum creatine kinase [Time Frame: 180 and 60 min before, and 60, 10, 180 and 300 min after training and protein intake]

Secondary ID(s)

Tine acute

Source(s) of Monetary Support

Please refer to primary and secondary sponsors

Secondary Sponsor(s)

The Research Council of Norway

Arkansas Children's Hospital Research Institute

Tine