Whole-exome Sequencing in Childhood Obesity

• Register: ClinicalTrials.gov
• Last refreshed on: 12 December 2020
• Main ID: NCT02418377
• Date of registration: 12/04/2015
• Prospective Registration: Yes
• Primary sponsor: National University, Singapore
• Public title: Whole-exome Sequencing in Childhood Obesity
• Scientific title: Whole-exome Sequencing to Identify Genetic Variants Associated With Severe Childhood Obesity, and Tracking the Changing Prevalence of Obesity Related Complications
• Date of first enrolment: August 10, 2015
• Target sample size: 800
• Recruitment status: Completed
• URL: https://clinicaltrials.gov/show/NCT02418377
• Study type: Observational [Patient Registry]

Countries of recruitment

Singapore

Contacts

• Name: Yung Seng Lee, M.D, PhD
• Affiliation: National University Health System

Key inclusion & exclusion criteria

Inclusion Criteria:

1.1. Inclusion Criteria

The obese subject must meet all of the following inclusion criteria to participate in this
study:

1. Ideal body weight for height (WFH) of at least 140% or BMI for age above 97th
percentile

2. Overweight before 10 years of age

3. Informed consent from both obese children subject and legal representative e.g.
parents

Family member must meet all of the following inclusion criteria to participate in this
study:

1. Must be parent or direct sibling of obese subject

2. Informed consent from both subject and parent is subject is below 21 years of age

Exclusion Criteria:

All subjects meeting any of the exclusion criteria at baseline will be excluded from
participation:

1. Unfit for blood testing and OGTT testing

2. No informed consent



Age minimum: N/A
Age maximum: N/A
Gender: All

Health Condition(s) or Problem(s) studied

Childhood Obesity

Primary Outcome(s)

Identify novel obesity genes and variants using whole-exome sequencing (WES) in our local Singaporean obese children. [Time Frame: 3 years]

Secondary Outcome(s)

Assess and compare the metabolic phenotype of the current severely obese children and severely obese children recruited in a similar fashion 15 years ago by the obesity gene study (OGS) group. [Time Frame: 3 years]

Biomarkers, inflammatory markers and PBMC gene expressions between metabolically healthy obese and metabolically unhealthy obese [Time Frame: 3 years]

Stool microbiota/metagenomics in obese children, and metabolic health [Time Frame: 3 years]

Secondary ID(s)

NMRC/CIRG/1407/2014

Source(s) of Monetary Support

Please refer to primary and secondary sponsors

Secondary Sponsor(s)

Genome Institute of Singapore A*Star, Singapore

Health Promotion Board, Singapore