Determining the acceptability and efficacy of new approaches for dengue vector control in Cambodia

• Register: ISRCTN
• Last refreshed on: 5 January 2021
• Main ID: ISRCTN85307778
• Date of registration: 26/10/2015
• Prospective Registration: No
• Primary sponsor: Malaria Consortium
• Public title: Determining the acceptability and efficacy of new approaches for dengue vector control in Cambodia
• Scientific title: Determining the acceptability and efficacy of guppies, pyriproxyfen, and community engagement on dengue vectors in Cambodia: A cluster randomized trial
• Date of first enrolment: 27/02/2015
• Target sample size: 21722
• Recruitment status: Completed
• URL: http://isrctn.com/ISRCTN85307778
• Study type: Interventional
• Study design: Three-arm cluster randomised controlled trial (Prevention)
• Phase: Not Applicable

Countries of recruitment

Cambodia

Contacts

• Name: John Hustedt

Key inclusion & exclusion criteria

Inclusion criteria: All households
Exclusion criteria: N/A

Age minimum:
Age maximum:
Gender: Both

Health Condition(s) or Problem(s) studied

Dengue fever
Infections and Infestations
Dengue fever

Intervention(s)

Public Randomization was selected as the method by which to randomize villages to evaluation arms. Village chiefs from all 30 clusters and HC chiefs from all seven HCs were invited to a central point (Prey Chhor Operational District) along with local and national authorities. Locally the concept of “lucky draw” is very popular, so we have decided to randomize the clusters based on this locally accepted method. Each representative from the cluster chose one rolled up paper labelled arm one, two, or three from a bowl. Three large sheets of butcher paper were put up on the wall labelled for each of the evaluation arms. As each representative selected their arm, project staff wrote the village name/s on the paper under that arm. After all clusters were chosen, project staff reviewed the three intervention arms and the role of the village chiefs and health centre staff in the project.

Arm 1: Guppy fish in key containers (>50 L) + direct PPF application (Sumilarv© 2MR) in containers (10-50 L) + COMBI
Arm 2. Guppy fish in key containers (>50 L) + COMBI;
Arm 3. Control (no intervention)

Guppies
The use of guppy fish (Poecilia reticulata) is a low cost, sustainable and effective approach to reduce dengue vector populations, and reduce the risk of dengue transmission. In Cambodia, more than 80% of the Aedes mosquito breeding is detected in key containers such as large water jars, cement tanks and other large containers used for the storage and collection of water for human and animal consumption and washing. Studies also pointed out that these large water jars were the most productive for mosquito pupae compared to other water storage containers and should be targeted for control. T

Primary Outcome(s)

Density of resting adult female Aedes agypti in the household as measured by entomology surveys at baseline, 4, 8 and 12 months.

Secondary Outcome(s)

1. Proportion of houses surveyed positive for Aedes aegypti larvae and/or pupae in any water container as measured by entomology surveys at baseline, 4, 8 and 12 months
2. Proportion of surveyed containers containing Aedes aegypti larvae and/or pupae as measured by entomology surveys at baseline, 4, 8 and 12 months
3. Number of containers positive for Aedes aegypti larvae and/or pupae per 100 houses surveyed as measured by entomology surveys at baseline, 4, 8 and 12 months
4. Number of pupae per household as measured by entomology surveys at baseline, 4, 8 and 12 months
5. Number of pupae per person as measured by entomology surveys at baseline, 4, 8 and 12 months
6. Percentage of wild caught mosquitos positive for dengue virus as measured by entomology surveys at baseline, 4, 8 and 12 months
7. Proportion of water containers with = 1 guppy fish as measured by entomology surveys at baseline, 4, 8 and 12 months and monthly monitoring by community volunteers
8. Proportion of water containers with at least 1 Sumilarv© 2MR as measured by entomology surveys at baseline, 4, 8 and 12 months and monthly monitoring by community volunteers
9. Percentage of households that have knowledge of mosquitos transmitting dengue and understand that they bite during the day as measured by a knowledge, attitudes, and practice survey at baseline and 12 months

Secondary ID(s)

N/A

Source(s) of Monetary Support

Department for International Development, German Society for International Cooperation (Deutsche Gesellschaft für Internationale Zusammenarbeit)

Secondary Sponsor(s)

London School of Hygene and Tropical Medicine

World Health Organization

Cambodian National Centre for Parasitology, Entomology, and Malaria Control

Ethics review

Status:
Approval date:
Contact:

1. Cambodian National Ethics Committee for Health Research, 09/10/2014, ref: 0285 2. London School of Hygiene and Tropical Medicine Observational/Interventions Research Ethics Committee, 03/06/2015, ref: 8812

Results

• Results available: Yes
• Date Completed: 31/12/2016