The content of sulphadoxine and pyrimethamine was determined by means of the HPLC method for the assay of sulphadoxine/pyrimethamine tablets described in the USP 24. The chromatographic parameters were as follows:
HPLC column: |
C18, 25cm × 4.6mm |
Mobile phase: |
80% glacial acetic acid (1:100), 20% acetonitrile, 500µl triethylamine |
Detection: |
UV at 254 nm |
Flow rate: |
2ml/min |
Injection volume: |
10µl |
The performance parameters were as follows:
Specificity: |
Specific for sulphadoxine and pyrimethamine as per USP |
Precision: |
%RSD<2 |
Linearity: |
>0.98 |
Peak symmetry: |
<1.9 |
Resolution: |
>1 |
No interfering peaks were observed during the analysis. The method was able to have indicated a counterfeit substance in some of the samples.
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