All sterilizers should be checked periodically. The sterilizer should be loaded in accordance with the manufacturer's instructions, with enough air space between packages to permit the proper circulation and penetration of steam or hot air. The effectiveness of sterilization should be regularly checked with biological indicators, autoclave control indicators or such other tests as may be devised to ensure that the contents of the load have been subjected to sterilization conditions.

Use of a new pattern of sterilizing box to contain needles is recommended. The box is made of a special kind of metal sensitive to heat, with air holes that open automatically under high temperature, and close when the temperature falls below 75°C.