The Quality of Antimalarials - A Study in Selected African Countries - EDM Research Series No. 030
(2003; 67 pages) View the PDF document
Table of Contents
View the documentAuthors
View the documentCountry Research Team Leaders
View the documentLaboratory Support
View the documentAcknowledgements
View the documentAcronyms
View the documentExecutive summary
View the document1. Background
Close this folder2. Methodology
Close this folder2.1 Analytical methods
View the document2.1.1 Method for the assay of chloroquine syrup
View the document2.1.2 Method for the assay of chloroquine tablets
View the document2.1.3 Method for the assay of sulphadoxine/pyrimethamine tablets
Open this folder and view contents2.2 Dissolution testing
View the document3. Results
Open this folder and view contents4. Discussion
Open this folder and view contents5. Conclusions and recommendations
View the documentReferences
Open this folder and view contentsTables
Open this folder and view contentsAnnexes
View the documentOther documents in the EDM Research Series
View the documentEDM Research Series No. 30
 

2.1.3 Method for the assay of sulphadoxine/pyrimethamine tablets

The content of sulphadoxine and pyrimethamine was determined by means of the HPLC method for the assay of sulphadoxine/pyrimethamine tablets described in the USP 24. The chromatographic parameters were as follows:

HPLC column:

C18, 25cm × 4.6mm

Mobile phase:

80% glacial acetic acid (1:100), 20% acetonitrile, 500µl triethylamine

Detection:

UV at 254 nm

Flow rate:

2ml/min

Injection volume:

10µl

The performance parameters were as follows:

Specificity:

Specific for sulphadoxine and pyrimethamine as per USP

Precision:

%RSD<2

Linearity:

>0.98

Peak symmetry:

<1.9

Resolution:

>1

No interfering peaks were observed during the analysis. The method was able to have indicated a counterfeit substance in some of the samples.

to previous section
to next section
 
 
The WHO Essential Medicines and Health Products Information Portal was designed and is maintained by Human Info NGO. Last updated: October 29, 2018