The Quality of Antimalarials - A Study in Selected African Countries - EDM Research Series No. 030
(2003; 67 pages) View the PDF document
Table of Contents
View the documentAuthors
View the documentCountry Research Team Leaders
View the documentLaboratory Support
View the documentAcknowledgements
View the documentAcronyms
View the documentExecutive summary
View the document1. Background
Close this folder2. Methodology
Close this folder2.1 Analytical methods
View the document2.1.1 Method for the assay of chloroquine syrup
View the document2.1.2 Method for the assay of chloroquine tablets
View the document2.1.3 Method for the assay of sulphadoxine/pyrimethamine tablets
Open this folder and view contents2.2 Dissolution testing
View the document3. Results
Open this folder and view contents4. Discussion
Open this folder and view contents5. Conclusions and recommendations
View the documentReferences
Open this folder and view contentsTables
Open this folder and view contentsAnnexes
View the documentOther documents in the EDM Research Series
View the documentEDM Research Series No. 30
 

2.1.2 Method for the assay of chloroquine tablets

The content of chloroquine was determined by UV-spectrophotometry in accordance with an adapted method for chloroquine phosphate tablets of the USP 24. The method consists of recording the UV absorbance of the sample at a wavelength of 343 nm and comparing it to the absorbance of a reference standard in a concentration range representing a 100% label claim at the same wavelength.

The method was adapted in such a way that it was possible to determine the quantity of the different salt forms of chloroquine in the tablets, where applicable. As all USP methods are specific for the analyte, the method was only validated in terms of precision. Interference from excipients was validated by superimposing the UV spectra of the sample and standard.

No interference from excipients was noted, and the precision of the method was %RSD < 2.

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