(nucleic acid based)
Company |
Abbott |
Roche |
Bayer |
bioMérieux |
bioMérieux |
Primagen |
Assay Name |
LcX® HIV RNA Quantitative |
Amplicor HIV-1 Monitor® Test |
Versant® HIV-1 RNA 3.0 Assay |
NucliSens® HIV-1-QT |
NucliSens EasyQ® HIV-1 |
RetinaTM Rainbow |
Type of assay |
RT-PCR |
RT-PCR |
bDNA |
NASBA |
NASBA |
NASBA |
Dynamic Range (copies/ml) |
50-1 000 000 |
50-750 000 |
75-500 000 |
50-1 000 000 |
50-3 000 000 |
500-50 000 000 |
Specimen Type |
Plasma |
Plasma, dried blood spots |
Plasma |
Plasma, serum, dried blood spots |
Plasma, serum, dried blood spots |
Plasma, serum, whole blood, dried blood spots |
Specimen volume |
200-1 000 µl |
100-500 µl |
1 000-2 000 µl |
10-2 000 µl |
10-2 000 µl |
200 µl |
Area of HIV genome amplified |
Pol |
Gag |
Pol |
Gag |
Gag |
LTR |
HIV-1 subtypes amplified |
Group M (subtypes A-G) and Group O |
All, plus some HIV-2 |
Group M (subtypes A-G) |
All |
All |
All |
Time for result |
5 hours |
6-7 hours |
22 hours |
2 hours |
2 hours |
1.5 hours |
Cost/test |
$40 |
$119 |
$125 |
$96 |
$60 |
$20 |
Number of samples/run |
21 (+3 controls) |
9-48 |
12-168 |
50 |
48 |
96 |
Equipment required14 |
Vaccuum pump Centrifuge (x2) Heat block LCx Analyser Thermal cycler |
COBAS Ampliprep Dead-air box Computer/printer Safety hood Heat block (x2) Centrifuge (x2) |
Bayer System 340 (bDNA Analyzer, Data Management Software, and computer system) Centrifuge Heatblock Waterbath Vaccuum system |
EasyQ Extractor Fume hood Waterbath Heat block (x4) EasyQ Reader Centrifuge (x3) Shaker |
EasyQ Extractor EasyQ Analyser |
RetinAlyser Heatblock Computer Centrifuge |
Equipment Cost ($US) |
8 500 + LCx Analyser 25 000 |
10 000 + COBAS Ampliprep 30 000 |
10 000 + Bayer System Analyzer |
140 000 |
130 000 |
23 000 |
14 All assays require pipettes, vortex mixers (& refrigerator for all but Primagen)
(non-nucleic acid based)
Company |
Cavidi |
Perkin Elmer |
Innogenetics |
Assay Name |
ExaVirTMLoad Quantitative HIV-RT Load Kit |
HIV-1 p24 Ultra ELISA ELAST ELISA amplification system |
InnotestTM |
Type of Assay |
Enzyme immunoassay for quantitation of RT activity |
Enzyme immunoassay for quantitation of p24 antigen |
Enzyme immunoassay for quantitation of p24 antigen |
Dynamic Range |
750-over 50 000 copies/ml |
400 copies/ml |
7 pg/ml -250 (50-200 000 copies/ml) |
Specimen Type |
Plasma |
Plasma, serum or cell culture supernatant |
Plasma, serum or cell culture supernatant |
Specimen Volume |
1000 µl |
100 µl |
100 µl |
Area of HIV Genome Selected for Amplification |
RT activity |
p24 antigen |
p24 antigen |
HIV-1 Subtypes Amplified |
All, plus HIV-2 |
HIV-1 |
All, plus HIV-2 |
Time for Result |
24 hours |
2.30 hours |
2 hours |
Cost/Test |
$13-15 |
$ 10 |
$10 |
Number of Samples/Run |
30 |
96 |
96 |
Equipment Required15 |
Incubator (33deg), Freezer, ELISA reader, computer |
Incubator, ELISA reader Refrigerator |
Incubator (37deg), ELISA reader, refrigerator |
Equipment Cost ($US) |
9 000-10 000 (start up pack includes other necessary equipment and 3 kits) |
7 000-9 000 |
7 000-9 000 |
15 All tests require pipettes and vortex mixers
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