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WHO Monographs on Selected Medicinal Plants - Volume 1
(1999; 295 pages) View the PDF document
Table of Contents
View the documentAcknowledgements
View the documentIntroduction
View the documentBulbus Allii Cepae
View the documentBulbus Allii Sativi
View the documentAloe
View the documentAloe Vera Gel
View the documentRadix Astragali
View the documentFructus Bruceae
View the documentRadix Bupleuri
View the documentHerba Centellae
View the documentFlos Chamomillae
View the documentCortex Cinnamomi
View the documentRhizoma Coptidis
View the documentRhizoma Curcumae Longae
View the documentRadix Echinaceae
View the documentHerba Echinaceae Purpureae
View the documentHerba Ephedrae
View the documentFolium Ginkgo
View the documentRadix Ginseng
View the documentRadix Glycyrrhizae
View the documentRadix Paeoniae
View the documentSemen Plantaginis
View the documentRadix Platycodi
View the documentRadix Rauwolfiae
View the documentRhizoma Rhei
View the documentFolium Sennae
View the documentFructus Sennae
View the documentHerba Thymi
View the documentRadix Valerianae
View the documentRhizoma Zingiberis
View the documentAnnex. Participants in the WHO Consultation on Selected Medicinal Plants
 

Radix Glycyrrhizae

Definition

Radix Glycyrrhizae consists of the dried roots and rhizomes of Glycyrrhiza glabra L. and its varieties (1–7) or of Glycyrrhiza uralensis Fisch. (6, 7) (Fabaceae).1

1 Glycyrrhiza inflata Bat. is listed in the Chinese pharmacopoeia (6). However, literature references to botanical, chemical, and biological studies on this species are rare. Therefore, it has not been included in this monograph.

Synonyms

Liquiritae officinalis Moench is a synonym of Glycyrrhiza glabra L. (1).

Selected vernacular names

Glycyrrhiza glabra L. and its varieties

Adimaduram, akarmanis, asloosoos, aslussos, athimaduram, athimaduramu, athimathuram, bekh-e-mahak, bois doux, cha em thet, estamee, gancao, glycyrrhiza, herbe aux tanneurs, hsi-pan-ya-kan-tsao, irk al hiel, irk al hilou, irksos, jakyakgamcho-tang, jashtimadhu, jethimadh, jethimadha, kanpo, kanzo, kan-ts'ao, kum cho, Lakritzenwurzel, licorice, licorice root, liquiritiae radix, liquorice, liquorice root, madhuyashti, madhuyashti rasayama, mulathee, muleti, mulhatti, neekhiyu, Persian licorice, racine de reglisse, racine douce, reglisse, reglisse officinalis, rhizoma glycyrrhizae, Russian licorice, Russian liquorice, Russisches Süssholz, si-pei, sinkiang licorice, Spanish licorice, Spanish liquorice, Spanisches Süssholz, Süssholzwurzel, sweet root, sweetwood, ud al sus, velmi, walmee, welmii, xi-bei, yashti, yashtimadhu, yashtimadhukam, yashtomadhu (115).

Glycyrrhiza uralensis Fisch.

Chinese licorice, Chinese liquorice, gancao, kan-ts'ao, kanzo, kanzoh, licorice root, liquiritiae radix, north-eastern Chinese licorice, saihokukanzoh, tohoku kanzo, tongpei licorice, tung-pei-kan-tsao, Ural liquorice, uraru-kanzo (1417).

Description

Glycyrrhiza glabra L. and its varieties

A perennial plant, up to more than 1m in height, erect, with highly developed stoloniferous roots. Leaves compound, 9–17 alternate imparipinnate leaflets, oblong to elliptical-lanceolate, acute or obtuse; racemes loose, shorter than the leaves or a little longer. Flowers 1 cm long. Flat pods oblong to linear, 1–3cm long by 6 mm wide, more or less densely echinate glandular, many-seeded or abbreviated, 2- or 3-seeded (1, 11).

Glycyrrhiza uralensis Fisch.

A perennial glandular herb, 30–100cm high. Stem erect, with short whitish hairs and echinate glandular hairs; the lower part of the stem is woody. Leaves alternate, imparipinnate; leaflets 7–17, ovate-elliptical, 2–5.5 cm long by 1–3cm wide; apex obtuse-rounded; base rounded; both surfaces covered with glandular hairs and short hairs. Stipules lanceolate. Inflorescence an axillary cluster. Flowers purplish, papilionaceous; calyx villous. Fruit a flat pod, oblong, sometimes falcate, 6–9mm wide, densely covered with brownish echinate glandular hairs. Seeds 2–8. The root is cylindrical, fibrous, flexible, 20–22cm long and 15mm in diameter, with or without cork, cork reddish, furrowed, light yellow inside (16).

Plant material of interest: dried root and rhizome

General appearance

Glycyrrhiza glabra L. and its varieties

The commercial variety, G. glabra var. typica Regel & Herd, known as Spanish liquorice, consists generally of roots and rhizomes in nearly cylindrical pieces, up to 1m long and 5–20mm in diameter; externally, the bark is brownish grey to dark brown, longitudinally wrinkled, occasionally bearing small dark buds in rhizomes or small circular or transverse rootlet-scars in roots. The peeled root is yellow, smooth, fibrous, finely striated; fracture, fibrous in the bark and splintery in the wood; internally, bright yellow. A distinct cambium ring separates the yellowish grey bark from the finely radiate yellow wood; central pith, only in rhizomes (1, 2, 7).

The commercial variety, G. glabra var. glandulifera (Wald et Kit) Regel & Herd, known as Russian liquorice, consists mainly of roots, in cylindrical pieces somewhat tapering and sometimes longitudinally split; 15–40cm long, 1–5cm in diameter. The enlarged crown of the root may attain up to 10 cm in diameter; externally, the unpeeled root purplish brown, somewhat scaly, with stem scars at the top; the peeled root yellowish, coarsely striated; fracture as for Spanish type; internally, yellow, radiating (1).

Glycyrrhiza uralensis Fisch.

The roots and rhizomes are cylindrical, fibrous, flexible, 20–100cm long, 0.6– 3.5 cm in diameter, with or without cork. Externally reddish brown or greyish brown, longitudinally wrinkled, furrowed, lenticellate, and with sparse rootlet scars. Texture compact, fracture slightly fibrous, yellowish white, starchy; cambium ring distinct, rays radiate, some with clefts. Rhizomes cylindrical, externally with bud scars, pith present in the centre of fracture (6, 7, 16, 17).

Organoleptic properties

Odour slight and characteristic (1, 6, 7); taste, very sweet (1, 6, 7, 13, 15, 17).

Microscopic characteristics

In transverse section the cork is thick, brown or purplish brown, formed of several layers of flattened polygonal thin-walled cells; cortex of phelloderm in root somewhat narrow, yellow fibres of parenchyma cells contain isolated prisms of calcium oxalate; phloem, wide, yellow, traversed by numerous wavy parenchymatous medullary rays, 1–8 cells wide and consisting of numerous radial groups of fibres, each surrounded by a crystal sheath of parenchyma cells. Each cell usually contains a prism of calcium oxalate and layers of parenchyma alternating with sieve tissue, the latter occasionally obliterated, appearing as refractive irregular structures; phloem fibres, very long, with very narrow lumen and strongly thickened stratified walls which are cellulosic in the inner part of the phloem and slightly lignified in the outer; xylem, yellow, distinctly radiate; xylem rays, consisting of small pale yellow parenchyma, groups of fibres similar to those of the phloem but more lignified, and surrounded by crystal-sheath, tracheids, and large wide lumen vessels, 80–200 µm in diameter, with thick yellow reticulate walls or with numerous oval bordered pits with slit-shaped openings. Other parenchyma cells contain small round or oval starch granules. Pith, only in rhizome, dark yellow, parenchymatous. Root, with 4-arch primary xylem, no pith and shows 4 broad primary medullary rays, radiating from the centre at right angles to one another. In peeled liquorice, the cork, cortex, and sometimes part of the phloem are absent (1).

Powdered plant material

Light yellow in the peeled or brownish yellow or purplish brown in the unpeeled root. Characterized by the numerous fragments of the fibres accompanied by crystal-sheath, the fibres 8–25 µm, mostly 10–15 µm, in diameter; dark yellow fragments of vessels, 80–200µm in diameter, containing solitary prismatic crystals of calcium oxalate, free or in cells 10–35µm (mostly 15–25µm) long; numerous simple oval, round or fusiform starch granules, free or in parenchyma cells, with no striation but occasionally showing hilum, 2– 20µm (mostly about 10µm) in diameter; cork may be present (1, 2, 7).

Geographical distribution

Glycyrrhiza glabra

Native to central and south-western Asia and the Mediterranean region (11, 12, 13). It is cultivated in the Mediterranean basin of Africa, in southern Europe, and in India (1, 11, 12, 13).

Glycyrrhiza uralensis

Northern China, Mongolia, and Siberia (16, 17).

General identity tests

Macroscopic, microscopic, and microchemical examinations (1–7); and thinlayer chromatographic analysis for the presence of glycyrrhizin (2–7).

Purity tests

Microbiology

The test for Salmonella spp. in Radix Glycyrrhizae products should be negative. The maximum acceptable limits of other microorganisms are as follows (18, 19, 20). For preparation of decoction: aerobic bacteria-not more than 107/g; fungi-not more than 105/g; Escherichia coli-not more than 102/g. Preparations for internal use: aerobic bacteria-not more than 105/g or ml; fungi-not more than 104/g or ml; enterobacteria and certain Gram-negative bacteria-not more than 103/g or ml; Escherichia coli-0/g or ml.

Total ash

Not more than 7% (6, 7).

Acid-insoluble ash

Not more than 2% (1–3, 6, 7).

Sulfated ash

Not more than 10% (2).

Water-soluble extractive

Not less than 20% (8).

Dilute alcohol-soluble extractive

Not less than 25% (7).

Pesticide residues

To be established in accordance with national requirements. Normally, the maximum residue limit of aldrin and dieldrin for Radix Glycyrrhizae is not more than 0.05 mg/kg (20). For other pesticides, see WHO guidelines on quality control methods for medicinal plants (18) guidelines for predicting dietary intake of pesticide residues (21).

Heavy metals

Recommended lead and cadmium levels are no more than 10 and 0.3mg/kg, respectively, in the final dosage form of the plant material (18).

Radioactive residues

For analysis of strontium-90, iodine-131, caesium-134, caesium-137, and plutonium-239, see WHO guidelines on quality control methods for medicinal plants (18).

Other purity tests

Alcohol-soluble extractive, chemical, and foreign organic matter tests to be established in accordance with national requirements.

Chemical assays

Assay for glycyrrhizin (glycyrrhizic acid, glycyrrhizinic acid) content (at least 4%) by means of spectrophotometric (1, 2), thin-layer chromatographic– densitometric (22, 23) or high-performance liquid chromatographic (24–26) methods.

Major chemical constituents

The major constituents are triterpene saponins. Glycyrrhizin (glycyrrhizic acid, glycyrrhizinic acid) is the major component (2–9%); minor components occur in proportions that vary depending on the species and geographical location (24–27). Glycyrrhizin occurs as a mixture of potassium and calcium salts (9). It is a monodesmoside, which on hydrolysis releases two molecules of D-glucuronic acid and the aglycone glycyrrhetic (glycyrrhetinic) acid (enoxolone) (28). Glycyrrhizin is generally regarded as the active principle of Radix Glycyrrhizae and is responsible for its sweetness, which is 50 times that of sucrose (27). Flavonoid constituents include liquiritigenin and isoliquiritigenin.

Dosage forms

Crude plant material, dried extract and liquid extract. Store in a well-closed container, protected from light and moisture (1, 3).

Medicinal uses

Uses supported by clinical data

None.

Uses described in pharmacopoeias and in traditional systems of medicine

As a demulcent in the treatment of sore throats, and as an expectorant in the treatment of coughs and bronchial catarrh. Also in the prophylaxis and treatment of gastric and duodenal ulcers, and dyspepsia (1, 6, 8, 27–29). As an anti-inflammatory agent in the treatment of allergic reactions (27), rheumatism and arthritis (9), to prevent liver toxicity, and to treat tuberculosis and adrenocorticoid insufficiency (9, 30).

Uses described in folk medicine, not supported by experimental or clinical data

As a laxative, emmenagogue, contraceptive, galactagogue, antiasthmatic drug, and antiviral agent (15). In the treatment of dental caries, kidney stones, heart disease (15), "consumption", epilepsy, loss of appetite, appendicitis, dizziness, tetanus, diphtheria, snake bite, and haemorrhoids (11, 13).

Pharmacology

Experimental pharmacology

The demulcent action of the drug is due primarily to glycyrrhizin (27). The antitussive and expectorant properties of the drug have also been attributed to glycyrrhizin, which accelerates tracheal mucus secretion (27).

The antiulcer activity of Radix Glycyrrhizae has been demonstrated both experimentally and clinically. Intraperitoneal, intraduodenal, or oral administration of aqueous or alcoholic extracts of Radix Glycyrrhizae reduced gastric secretions in rats, and it inhibited the formation of gastric ulcers induced by pyloric ligation, aspirin, and ibuprofen (27, 31–32). Glycyrrhizin and its agly- cone (glycyrrhetic acid, enoxolone), two of the active constituents of Radix Glycyrrhizae, both have antiphlogistic activity and increase the rate of mucus secretion by the gastric mucosa (9). Deglycyrrhizinated liquorice (97% of glycyrrhizin is removed) effectively treated stress-induced ulcers in animal models (31–34). The mechanism of antiulcer activity involves acceleration of mucin excretion through increasing the synthesis of glycoprotein at the gastric mucosa, prolonging the life of the epithelial cells, and antipepsin activity (32).

The spasmolytic activity of Radix Glycyrrhizae has been demonstrated in vivo (guinea-pig, rabbit, and dog) (35–37), and appears to be due to the flavonoids liquiritigenin and isoliquiritigenin (38).

Glycyrrhizin reduces the toxic action of carbon tetrachloride- and galactosamine-induced cytotoxicity in cultured rat hepatocytes, through its antioxidant activity (9, 27). Glycyrrhizin inhibited histamine release from rat mast cells and prevented carbon tetrachloride-induced liver lesions and macrophage-mediated cytotoxicity (27). Intragastric administration of a flavonoid fraction isolated from Radix Glycyrrhizae to mice protected against carbon tetrachloride hepatotoxicity (39). Glycyrrhizin protected the liver apparently through its membrane stabilization effects (27).

The anti-inflammatory and antiallergic actions of the drug have been attributed to the corticosteroid-like activity of glycyrrhizin and glycyrrhetic acid (enoxolone). These compounds act indirectly by potentiating the activity of corticosteroids. In vitro, glycyrrhetic acid inhibits Δ4β-reductase, an enzyme that competitively inactivates steroid hormones, and 11β-hydroxysteroid dehydrogenase, the enzyme that deactivates cortisol (27). Glycyrrhizin given intraperitoneally suppressed contact dermatitis in mice, and was more effective than prednisolone, but no effects were observed after oral administration (9).

In vitro, the drug inhibits the growth of Bacillus subtilis (40), Mycobacterium tuberculosis (41), Aspergillus spp. (42), Staphylococcus aureus, Mycobacterium smegmatis, and Candida albicans (43).

Clinical pharmacology

Oral administration of Radix Glycyrrhizae to 15 patients with peptic ulcer reduced symptoms and improved healing in 75% of the cases (44). Glycyrrhetic acid (enoxolone), the active constituent, produced its antiulcer activity by inhibiting 15-hydroxyprostaglandin dehydrogenase and Δ13-prostaglandin reductase (45). Inhibition of these two enzymes stimulated an increase in the concentration of prostaglandins E and F in the stomach, which promoted the healing of peptic ulcers owing to a cytoprotective effect on the gastric mucosa (45). Carbenoxolone, a derivative of glycyrrhetic acid, has been used clinically for years in the treatment of gastric and duodenal ulcers (46).

Oral administration of deglycyrrhizinated liquorice (380 mg, 3 times daily) to 169 patients with chronic duodenal ulcers was as effective as antacid or cimetidine treatments (47). These results indicate that, in addition to glycyrrhetic acid, other unidentified constituents of Radix Glycyrrhizae contribute to its antiulcer activity.

Reports on the usefulness of liquorice extracts on body fluid homeostasis in patients with Addison disease are contradictory. One study found no positive effects (48), while three other studies noted an increase in weight gain and sodium retention (49–51).

Contraindications

Radix Glycyrrhizae is contraindicated in patients with hypertension, cholestatic disorders or cirrhosis of the liver, hypokalaemia, or chronic renal insufficiency, and during pregnancy (9, 29).

Warnings

Prolonged use of large doses (>50g/day) of the drug for extended periods (>6 weeks) may increase water accumulation, causing swelling of the hands and feet. Sodium excretion is reduced and potassium excretion is increased. Blood pressure may rise.

Precautions

General

Radix Glycyrrhizae should not be taken concurrently with corticosteroid treatment. If sore throat or cough persists for more than 3 days, the patient should consult a physician.

Drug interactions

Because it increases potassium loss, Radix Glycyrrhizae should not be administered for prolonged use with thiazide and loop diuretics or cardiac glycosides (29). Because it reduces sodium and water excretion, the effectiveness of drugs used in the treatment of hypertension may be reduced. Radix Glycyrrhizae should not be administered in conjunction with spironolactone or amiloride (52).

Carcinogenesis, mutagenesis, impairment of fertility

Radix Glycyrrhizae is not mutagenic in vitro (53–55).

Pregnancy: teratogenic effects

The drug is not teratogenic in animal models (56).

Pregnancy: non-teratogenic effects

The safety of Radix Glycyrrhizae preparations during pregnancy has not been established. As a precautionary measure the drug should not be used during pregnancy.

Nursing mothers

The safety of Radix Glycyrrhizae preparations during lactation has not been established. As a precautionary measure the drug should not be used during lactation except on medical advice.

Paediatric use

The safety and effectiveness of the drug in children have not been established.

Other precautions

No information available about drug and laboratory test interactions.

Adverse reactions

No adverse reactions have been associated with the drug when used within the recommended dosage and treatment period.

Prolonged use (>6 weeks) of excessive doses (>50g/day) can lead to pseudoaldosteronism, which includes potassium depletion, sodium retention, oedema, hypertension, and weight gain (9, 57, 58). In rare cases, myoglobinuria and myopathy can occur (59).

Posology

Unless otherwise prescribed, average daily dose of crude plant material, 5–15g, corresponding to 200–800mg of glycyrrhizin. Doses of other preparations should be calculated accordingly (29). Radix Glycyrrhizae should not be used for longer than 4–6 weeks without medical advice.

References

1. African pharmacopoeia, Vol. 1, 1st. ed. Lagos, Organization of African Unity, Scientific Technical & Research Commission, 1985:131–134.

2. European pharmacopoeia, 2nd ed. Strasbourg, Council of Europe, 1995.

3. British pharmacopoeia. London, Her Majesty's Stationery Office, 1988.

4. Deutsches Arzneibuch 1996. Stuttgart, Deutscher Apotheker Verlag, 1996.

5. Pharmacopoeia helvetica VII. Berne, Département fédéral de l'intérieur, 1994.

6. Pharmacopoeia of the People's Republic of China (English ed.). Guangzhou, Guangdong Science and Technology Press, 1992.

7. The pharmacopoeia of Japan XII. Tokyo, The Society of Japanese Pharmacopoeia, 1991.

8. Farmakope Indonesia, 4th ed. Jakarta, Departemen Kesehatan, Republik Indonesia, 1995.

9. Bradley PR, ed. British herbal compendium, Vol. 1. Bournemouth, British Herbal Medicine Association, 1992:145–148.

10. Kapoor LD. Handbook of Ayurvedic medicinal plants. Boca Raton, FL, CRC Press, 1990:194–195.

11. The Indian pharmaceutical codex. Vol. I. Indigenous drugs. New Delhi, Council of Scientific & Industrial Research, 1953:112–113.

12. Ghazanfar SA. Handbook of Arabian medicinal plants. Boca Raton, FL, CRC Press, 1994:110–111.

13. Chin WY, Keng H. An illustrated dictionary of Chinese medicinal herbs. Singapore, CRCS Publications, 1992.

14. Hsu HY. Oriental materia medica, a concise guide. Long Beach, CA, Oriental Healing Arts Institute, 1986:532–535.

15. Farnsworth NR, ed. NAPRALERT database. University of Illinois at Chicago, IL, August 21, 1995 production (an on-line database available directly through the University of Illinois at Chicago or through the Scientific and Technical Network (STN) of Chemical Abstracts Services).

16. Medicinal plants in China. Manila, World Health Organization, 1989 (WHO Regional Publications, Western Pacific Series, No. 2).

17. Keys JD. Chinese herbs, their botany, chemistry and pharmacodynamics. Rutland, VT, CE Tuttle, 1976:120–121.

18. Quality control methods for medicinal plant materials. Geneva, World Health Organization, 1998.

19. Deutsches Arzneibuch 1996. Vol. 2. Methoden der Biologie. Stuttgart, Deutscher Apotheker Verlag, 1996.

20. European pharmacopoeia, 3rd ed. Strasbourg, Council of Europe, 1997.

21. Guidelines for predicting dietary intake of pesticide residues, 2nd rev. ed. Geneva, World Health Organization, 1997 (unpublished document WHO/FSF/FOS/97.7; available from Food Safety, WHO, 1211 Geneva 27, Switzerland).

22. Takino Y et al. Quantitative determination of glycyrrhizic acid in liquorice roots by TLC-densitometry studies on the evaluation of crude drugs. VI. Planta medica, 1979, 36:74–78.

23. Vanhaelen M, Vanhaelen-Fastré R. Quantitative determination of biologically active constituents in medicinal plant crude extracts by thin-layer chromatography densitometry. Journal of chromatography, 1983, 281:263–271.

24. Sticher O, Soldati F. Glycyrrhizinsäure-Bestimmung in Radix Liquiritiae mit Hochleistungs-flüssigkeitschromatographie (HPLC). Pharmaceutica acta Helvetica, 1978, 53:46–52.

25. Sagara K. Determination of glycyrrhizin in pharmaceutical preparations by ionpair high-performance liquid chromatography. Shoyakugaku zasshi, 1986, 40:77– 83.

26. Okada K et al. High-speed liquid chromatographic analysis of constituents in licorice root. I. Determination of glycyrrhizin. Yakugaku zasshi, 1981, 101:822–828.

27. Hikino H. Recent research on Oriental medicinal plants. In: Wagner H, Hikino H, Farnsworth NR, eds. Economic and medicinal plant research. Vol. 1. London, Academic Press, 1985:53–85.

28. Bruneton J. Pharmacognosy, phytochemistry, medicinal plants. Paris, Lavoisier, 1995:549– 554.

29. German Commission E Monograph, Liquiritiae radix. Bundesanzeiger, 1985, 90:15 May

30. Schambelan M. Licorice ingestion and blood pressure regulating hormones. Steroids, 1994, 59:127–130.

31. Dehpour AR et al. The protective effect of liquorice components and their derivatives against gastric ulcer induced by aspirin in rats. Journal of pharmacy and pharmacology, 1994, 46:148–149.

32. Dehpour AR et al. Antiulcer activities of liquorice and its derivatives in experimental gastric lesion induced by ibuprofen in rats. International journal of pharmaceutics, 1995, 119:133–138.

33. Morgan RJ et al. The protective effect of deglycyrrhinized liquorice against aspirin and aspirin plus bile acid-induced gastric mucosal damage, and its influence on aspirin absorption in rats. Journal of pharmacy and pharmacology, 1983, 35:605– 607.

34. Russell RI, Morgan RJ, Nelson LM. Studies on the protective effect of deglycyrrhinized liquorice against aspirin (ASA) and ASA plus bile acid-induced gastric mucosal damage, and ASA absorption in rats. Scandinavian journal of gastroenterology, 1984, 19(Suppl.):97–100.

35. Takagi K, Harada M. Pharmacological studies on herb Peony root. III. Effects of peoniflorin on circulatory and respiration system and isolated organs. Yakugaku zasshi, 1969, 89:893–896.

36. Wrocinski T. Determination of the activity of spasmolytic drugs with reference to the papaverine standard. Biuletyn Instytutu Roslin Leczniczych, 1960, 6:236.

37. Shihata M, Elghamry MI. Experimental studies in the effect of Glycyrrhiza glabra. Planta medica, 1963, 11:37.

38. Chandler RF. Licorice, more than just a flavour. Canadian pharmaceutical journal, 1985, 118:420–424.

39. Wang GS, Han ZW. The protective action of Glycyrrhiza flavonoids against tetrachloride hepatotoxicity in mice. Yao hsueh hsueh pao, 1993, 28:572–576.

40. Sabahi T et al. Screening of plants from the southeast of Iran for antimicrobial activity. International journal of crude drug research, 1987, 25:72–76.

41. Grange JM, Davey RW. Detection of antituberculous activity in plant extracts. Journal of applied bacteriology, 1990, 68:587–591.

42. Toanun C, Sommart T, Rakvidhyasastra V. Effect of some medicinal plants and spices on growth of Aspergillus. Proceedings of the 11th Conference of Science and Technology. Bangkok, Kasetsart University, 1985:364–365.

43. Mitscher LA et al. Antimicrobial agents from higher plants. Antimicrobial isoflavonoids and related substances from Glycyrrhiza glabra L. var. typica. Journal of natural products, 1980, 43:259–269.

44. Chaturvedi GN. Some clinical and experimental studies on whole root of Glycyrrhiza glabra L. (Yashtimadhu) in peptic ulcer. Indian medical gazette, 1979, 113:200– 205.

45. Baker ME, Fanestil DD. Liquorice as a regulator of steroid and prostaglandin metabolism. Lancet, 1991, 337:428–429.

46. Rask-Madsen J et al. Effect of carbenoxolone on gastric prostaglandin E2 levels in patients with peptic ulcer disease following vagal and pentagastrin stimulation. European journal of clinical investigation, 1983, 13:875–884.

47. Kassir ZA. Endoscopic controlled trial of four drug regimens in the treatment of chronic duodenal ulceration. Irish medical journal, 1985, 78:153–156.

48. Molhuysen JA et al. A liquorice extract with deoxycortone-like action. Lancet, 1950, ii:381–386.

49. Groen J et al. Extract of licorice for the treatment of Addison's disease. New England journal of medicine, 1951, 244:471–475.

50. Card WI et al. Effects of liquorice and its derivatives on salt and water metabolism. Lancet, 1953, i:663–667.

51. Groen J et al. Effect of glycyrrhizinic acid on the electrolyte metabolism in Addison's disease. Journal of clinical investigation, 1952, 31:87–91.

52. Doll R. Treatment of gastric ulcer with carbenoxolone: antagonistic effect of spironolactone. Gut, 1968, 9:42–45.

53. Sakai Y et al. Effects of medicinal plant extracts from Chinese herbal medicines on the mutagenic activity of benzo[a]pyrene. Mutation research, 1988, 206:327–334.

54. Lee HK et al. Effect of bacterial growth-inhibiting ingredients on the Ames mutagenicity of medicinal herbs. Mutation research, 1987, 192:99–104.

55. Yamamoto H, Mizutani T, Nomura H. Studies on the mutagenicity of crude drug extracts. I. Yakugaku zasshi, 1982, 102:596–601.

56. Leslie GB, Salmon G. Repeated dose toxicity studies and reproductive studies on nine Bio-Strath herbal remedies. Swiss medicine, 1979, 1:1–3.

57. Epistein MT et al. Effects of eating liquorice on the renin-angiotensin aldosterone axis in normal subjects. British medical journal, 1977, 1:488–490.

58. Stewart PM et al. Mineralocorticoid activity of liquorice: 11-β hydroxysteroid dehydrogenase deficiency comes of age. Lancet, 1987, ii:821–824.

59. Caradonna P et al. Acute myopathy associated with chronic licorice ingestion: Reversible loss of myoadenylate deaminase activity. Ultrastructural pathology, 1992, 16:529–535.

 

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