The Nile Treatment Centre (NTC) which opened in January 2004 is a nongovernmental agency located on the Jinja to Kampala highway, 5 kilometres from Jinja. The location makes it less accessible than the public facility and allows for greater privacy. NTC is funded by PEPFAR and is under the aegis of the Uganda Business Coalition against HIV/AIDS, which has two other centres in Kampala.

NTC has 12 staff members: three doctors, two counsellors, two phlebotomists, one dispenser, one pharmacist, two nurses, and one medical assistant who offer services to PLWHIV. It is open five days a week and the staff work a half-day on Saturdays. The Centre receives 30 patients a day on average and the doctor patient ratio is 1:15, with two doctors on duty at any one time. Like JRRH, NTC follows the National Antiretroviral Treatment and Care Guidelines and offers similar regimens.

NTC opens at 0830 hours. On arrival patients are welcomed into comfortable seats and welcomed by a receptionist who then asks for the patient's confidential number. The receptionist keys this number into the computer and checks whether the patient has come for their appointment on the correct day. She then refers the patient to the health workers they are scheduled to meet that day.

For all new patients, the importance of near-perfect adherence is emphasized by the adherence counsellor on the first visit. This counselling is carried out in groups or individually on request. Patients are then sent to the doctor, who assesses the patient's condition and requests blood or other tests, including a repeat HIV test. Prior to testing, the phlebotomist checks that the client is aware of the critical importance of adherence. A similar check is carried out by the pharmacist before releasing medicines to any patient.