(1997; 248 pages) [French]
A floor space of 300-400 m2 is required. All laboratory rooms should be supplied with running water and drainage, electrical power, and gas (either centrally supplied or from a gas tank). Climatic conditions will determine the need for air-conditioning and heating systems. The supply of water should be of adequate pressure for the use of vacuum aspirators (at least 19 kPa or 20 N/cm2 are needed), otherwise suitable vacuum pumps should be installed. An arrangement to recirculate water used by vacuum aspirators through a collection tank may considerably reduce total water needs and should be considered if the water supply is scarce or irregular. A sewage treatment installation should also be provided (e.g. a lime pit to neutralize acidic effluents). The building should be constructed of fire-resistant material and the layout of the modules and connecting corridors should be determined not only by working efficiency but also by safety considerations, particularly in areas where inflammable liquids or compressed gases are used or stored. If large quantities of inflammable reagents are to be stored, the space should be planned and constructed in accordance with local fire regulations.
Each unit should be provided with rooms equipped for its specific requirements, including hooded benches in chemical rooms; ample electrical outlets in physicochemical rooms and voltage-stabilizing equipment if the local power supply is variable; movement-damping tables in balance rooms; laminar airflow equipment in microbiological rooms. All rooms should be provided with storage cabinets for reagents, glassware, and samples, wall shelving, and writing desks.
Control of temperature and humidity of at least a part of the laboratory area is imperative in tropical regions. In particular, the room for chromatographic work (primarily thin-layer chromatography) should be thermostatically controlled and in all cases protected from draughts and direct sunlight. Rooms equipped with hoods and extractor fans should receive an inflow of dry, cool air and additional dehumidifiers are required in storage areas for reference materials and samples.
Rabbits used for pyrogen testing should be kept in a room apart from other areas of the laboratory. A separate unit should be provided if other experiments on animals are contemplated. Both the animal house and the animal experimentation rooms should be thermostatically controlled within +2 °C. In warmer climates the temperature is usually maintained within the range 23-25 °C.
Advice on technical facilities required for microbiological testing is provided in the twenty-second report of the WHO Expert Committee on Biological Standardization.1
1 WHO Technical Report Series, No. 444, 1970.