Accelerated Degradation Experiment
Conditions
Procedure A: |
30 days exposure at 50 °C and 100% relative humidity. |
Procedure B: |
30 days exposure at 50 °C and 100% relative humidity followed by 5 days at 70 °C and 100% relative humidity. |
Appearance of the substance
The substance, originally in the form of fine white or very slightly pink needles, changes after procedure A into a liquid that solidifies on cooling, resulting to a dark pink mass, and after procedure B into a liquid that solidifies on cooling, giving a light red-brown mass.
Gain of moisture
After procedure B: 6%
Melting point
Before exposure to heat and humidity |
62 °C |
After procedure B: |
60 °C |
Chromatographic test
Adsorbent: Silica gel F-254
Solution applied to the plate: Substance dissolved in methanol.
Solvent system:
chloroform: .........................8 volumes
ethane 95%: .......................2 volumes
Development conditions: The solvent is allowed to migrate until the front reaches a line 15 cm from the starting line.
Detection: The plate is left in a chromatographic chamber containing some iodine crystals until spots appear.
Results: The spots obtained from the solution of each of the samples subjected to procedures A and B and the spots obtained from the reference solution of hexylresorcinol display the same chromatographic characteristics of migration and detection.
Conclusion
No decomposition is detected by thin-layer chromatography, but after procedures A and B, the appearance of the substance changes.
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