Accelerated Degradation Experiment
Conditions
Procedure A: |
30 days exposure at 50 °C and 100% relative humidity. |
Procedure B: |
30 days exposure at 50 °C and 100% relative humidity followed by 7 days at 70 °C and 100% relative humidity. |
Appearance of the substance
The substance, originally in the form of a white or almost white, crystalline powder, changes after procedure A into a white, humid mass and after procedure B into two phases, an upper clear liquid one, and a lower one as a white, solid mass.
Gain of moisture
Before exposure to heat and humidity: |
9% |
After procedures A and B: |
40% |
Chromatographic test
Adsorbent: Cellulose
Solution applied to the plate: Substance dissolved in methanol.
Solvent system:
2-propanol ................... 6 volumes
water ............................ 3 volumes
acetic acid .................... 1 volume
Development conditions: The solvent is allowed to migrate until the front reaches a line 15 cm from the starting line.
Detection: The plate is left in a chromatographic chamber containing some iodine crystals until spots appear.
Results: The spots obtained from the solution of the sample subjected to procedure A and the spot obtained from the reference solution of suxamethonium chloride display the same chromatographic characteristics of migration and detection. After procedure B, an additional spot is observed which is more mobile than the main spot.
Conclusion
Suxamethonium chloride is subject to decomposition only at higher temperatures as seen after procedure B.
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