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Basic Tests for Drugs - Pharmaceutical Substances, Medicinal Plant Materials and Dosage Forms
(1998; 100 pages) [French] [Spanish] View the PDF document
Table of Contents
View the documentPreface
View the document1. Introduction
View the document2. Other collections of simple tests
View the document3. Test procedures for pharmaceutical substances
View the document4. Test procedures for medicinal plant materials
View the document5. Test procedures for pharmaceutical dosage forms
View the document6. Reagents
View the documentAcknowledgements
View the documentOther WHO publications on pharmaceuticals
View the documentBack cover

6. Reagents

Most of the reagents, test solutions and volumetric solutions needed to perform the test procedures outlined in sections 4-6 of this manual are described in Basic tests for pharmaceutical substances and/or Basic tests for pharmaceutical dosage forms and only additional reagents are given below. Reagents are denoted by the abbreviation R, test solutions by the abbreviation TS, and volumetric solutions by the abbreviation VS. The concentration of the reagent solutions is expressed in g/l, that is, grams of anhydrous substance per litre of water or solvent, as indicated. Where no solvent is indicated, demineralized water should be used. The procedures for the preparation of test solutions that require special attention are given in detail.

Ammonium acetate R.

Ammonium mercurithiocyanate TS.

Procedure. Dissolve 30 g of ammonium thiocyanate R and 27 g of mercuric chloride R in sufficient water to produce 1000 ml.

Ammonium vanadate R.

Ammonium vanadate (5 g/l) TS.

Amyl alcohol R.

Anthrone R.

Anthrone TS.

Procedure. Dissolve 35 mg of anthrone R in 100 ml of sulfuric acid (~1760 g/l) TS.

Catechol R.

Ceric ammonium nitrate R.

Ceric ammonium nitrate TS.

Procedure. Dissolve 6.25 g of ceric ammonium nitrate R in 10 ml of nitric acid (15 g/l) TS.

Shelf life. Use the solution within 3 days.

Chloramine B R.

Chloramine B (50 g/l) TS.

Note. Chloramine B (50 g/l) TS must be freshly prepared.

Cobalt(II) nitrate (10 g/l) TS.

Copper(II) sulfate (1 g/l) TS.

Diazobenzenesulfonic acid TS2.

Procedure. To 0.2 g of sulfanilic acid R add 20 ml of hydrochloric acid (0.1 mol/l) VS, boil to dissolve, cool to 4°C in ice, add drop by drop while stirring 0.9 ml of sodium nitrite (100 g/l) TS and allow to stand in ice for 10 minutes. Then add 0.5 ml of sulfamic acid (100 g/l) TS.

Note. Diazobenzenesulfonic acid TS2 must be freshly prepared.

Ethanol (~375 g/l) TS.

Iodine (0.05 mol/l) VS.

Procedure. Dissolve 1.27 g of iodine R and 1.80 g of potassium iodide R in sufficient water to produce 100 ml.

Lanthanum nitrate R.

Lanthanum nitrate (30 g/l) TS.

Procedure. Dissolve 4.3 g of lanthanum nitrate R in 1 ml of nitric acid (~130 g/l) TS and sufficient water to produce 100 ml.

N-(1-Naphthyl)ethylenediamine hydrochloride R.

N-(1-Naphthyl)ethylenediamine/ethanol TS.

Procedure. Dissolve 0.10 g of N-(1-naphthyl)ethylenediamine hydrochloride R in a mixture of equal volumes of ethanol (~750 g/l) TS and water.

Nitric acid (15 g/l) TS.

Nitrobenzene R.

Phosphomolybdic acid R.

Phosphomolybdic acid/ethanol TS.

Procedure. Dissolve 5 g of phosphomolybdic acid R in sufficient dehydrated ethanol R to produce 100 ml.

Phosphotungstic acid R.

Phosphotungstic acid (10 g/l) TS.

Potassium carbonate (100 g/l) TS.

Potassium chloride R.

Potassium chloride (100 g/l) TS.

Potassium hydroxide (~110 g/l) TS.

Potassium hydroxide (~55 g/l) TS.

Potassium nitrite R.

Potassium nitrite (100 g/l) TS.

2-Propanol R.

Resorcinol (20 g/l) TS.

Sodium chloride (100 g/l) TS.

Sodium nitrite (500 g/l) TS.

Sodium nitrite (100 g/l) TS.

Sodium nitroprusside R.

Sodium nitroprusside (45 g/l) TS.

Note. Sodium nitroprusside (45 g/l) TS must be freshly prepared.

Sucrose R.

Sucrose/hydrochloric acid TS.

Procedure. Dissolve about 0.1 g of sucrose R in 10 ml of hydrochloric acid (~420 g/l) TS.

Tetramethylammonium hydroxide (~100 g/l) TS.

Tetramethylammonium hydroxide/ethanol TS.

Procedure. Dilute 10 ml of tetramethylammonium hydroxide (~100 g/l) TS with sufficient ethanol (~750 g/l) TS to produce 100 ml.

Tin(II) chloride R.

Titan yellow R.

Titan yellow TS.

Procedure. Dissolve 0.05 g of titan yellow R in sufficient water to produce 100 ml.

Tosylchloramide sodium R.

Tosylchloramide sodium (40 g/l) TS.

Triketohydrindene/butanol TS.

Procedure. Dissolve 0.2 g of triketohydrindene hydrate R in sufficient 1-butanol R to produce 100 ml.

Triphenyltetrazolium chloride R.

Triphenyltetrazolium chloride/ethanol TS.

Procedure. Dissolve 0.5 g of triphenyltetrazolium chloride R in sufficient dehydrated ethanol R to produce 100 ml.


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