Enumeration of CD4 T lymphocytes : report of a workshop, Bangkok, Thailand, 6-10 June 2006
AbstractDescription: Enumeration of CD4 T lymphocytes is essential for initiation of antiretroviral therapy for a person with HIV as well as for monitoring the response to treatment. The techniques for enumeration of CD4 T lymphocytes are evolving. The flow cytometry continues to be the gold standard of CD4 T lymphocytes enumeration in spite of the fact that the technology is expensive both in terms of initial investment in sophisticated equipment and running cost. The newer techniques may be economical to procure and maintain. The latter are particularly intended for countries with limited resources. WHO conducted a workshop to orient the nationals about these technologies and integration of a quality system into routine testing, at Siriraj Hospital Bangkok. The report describes the status of CD4 testing in these countries. While Indonesia, India and Thailand have developed a good infrastructure for enumeration of CD4 T lymphocytes, Bangladesh, Myanmar, Nepal and Sri Lanka have made a beginning in this direction. The workshop focussed on principles of various techniques and imparted a hands-on training for performing the same. The issues regarding selection of technology, procurement of equipment and their maintenance; ensuring continuous supply and economical use of reagents and biosafety in laboratory were discussed and have been presented in this Report.
World Health Organization, Regional Office for South-East Asia. (2006). Enumeration of CD4 T lymphocytes : report of a workshop, Bangkok, Thailand, 6-10 June 2006. WHO Regional Office for South-East Asia. http://www.who.int/iris/handle/10665/205022