Establishment of PCR laboratory in developing countries
AbstractPolymerase chain reaction (PCR) technology has become the mainstay of molecular diagnosis of several infectious diseases. This manual is designed to offer a basic knowledge of the principles and utility of PCR- based assays for diagnosis of viral infections. It includes a few protocols of commercially available nucleic acid extraction kits and PCR assays, as well as the protocols of PCR assays developed, standardized and available for routine diagnosis. This manual provides primary guidelines to assist clinical and laboratory personnel in developing countries to establish a PCR diagnostic facility and expand its diagnostic profile for efficient support to patient care as well as public health actions.
World Health Organization, Regional Office for South-East Asia. (2011). Establishment of PCR laboratory in developing countries. WHO Regional Office for South-East Asia. http://www.who.int/iris/handle/10665/205020