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Title: Detection of transovarial dengue virus from field-caught Aedes aegypti and Ae. albopictus larvae using C6/36 cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques.
Authors: Rohani, A
Zamree, I
Lee, H L.
Mustafakamal, I
Norjaiza, M J
Kamilan, D
Issue Date: 2007
Publisher: WHO Regional Office for South-East Asia.
Place of publication: New Delhi
Language: English
Abstract: Larvae of Aedes aegypti and Aedes albopictus were collected from a wide variety of artificial containers. Most samples were collected from used tyres and water-holding containers located in residential urban or rural areas. The identified mosquito larvae were pooled according to the species, date and locality and stored at –70 °C. A total of 378 pools of Ae. aegypti and 553 pools of Ae. albopictus were collected. Virus isolation was carried out using cell culture (C6/36 clone) of Ae. albopictus and virus detection by reverse-transcriptase polymerase chain reaction (RT-PCR). Transovarial transmission of dengue virus was demonstrated in both Ae. aegypti and Ae. albopictus in nature. Infected larvae were recovered from 16 localities (10 in Terengganu; 5 in Kuala Lumpur and 1 in Pahang). The study showed that both the cell culture and RT-PCR techniques can be used to detect dengue virus from mosquito larvae.
Description: Dengue Bulletin. 2007 Dec; 31: 47-57.
Keywords: Dengue
Aedes Albopictus
Aedes Aegypti
ISSN: 0250-8362
Appears in Collections:Dengue Bulletin

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