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Title: Evaluation of the COBAS AMPLICOR MTB test for the detection of Mycobacterium tuberculosis complex
Authors: Abu Amero, K.K.
Halablab, M.A.
Issue Date: 2004
Language: English
Abstract: We evaluated the COBAS AMPLICOR polymerase chain reaction [PCR] based test for the detection of Mycobacterium tuberculosis complex in 866 respiratory and non-respiratory samples. Acid-fast staining and culture on Lowenstein-Jensen medium were also performed on all samples. Of the 866 samples tested, 87 [10.0%] were PCR-positive compared to 94 [10.9%] culture positive. There were no false positive results but 7 PCR-negative, culture-positive samples were, considered false negatives after reviewing medical records of patients. A PCR inhibitory rate of 2.0% [17/866] was observed in respiratory samples only. Sensitivity, specificity, and positive and negative predictive values for this test were 92.5%, 100%, 100% and 99.1% respectively. This test is a valuable diagnostic tool for today's mycobacteriology laboratory
Description: 329-335
Keywords: Biopsy
Bronchoalveolar Lavage Fluid
Cell Culture Techniques
Cerebrospinal Fluid
Coloring Agents
False Positive Reactions
Sensitivity and Specificity
Subject: DNA, Bacterial
ISSN: 1020-3397
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Appears in Collections:EMRO Journal Articles (EMHJ)

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