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Title: Antimony-resistant Leishmania donovani in eastern Sudan: incidence and in vitro correlation
Authors: Abdo, M.G.
El Amin, W.M.
Khalil, E.A.G.
Mukhtar, M.M.
Issue Date: 2003
Language: English
Abstract: A longitudinal study was done in a leishmaniasis -endemic region in eastern Sudan during the period November 2001-February 2003 to determine the incidence of failure of sodium stibogluconate treatment. We studied 820 confirmed visceral leishmaniasis patients. All were treated with sodium stibogluconate, 20 mg/kg body weight for at least 28 days. Parasites were isolated from lymph node aspirates from 22 participants identified as relapsed patients. All isolates were typed as Leishmania donovani based on polymerase chain reaction [PCR] amplification of parasite kDNA. Six parasites showed in vitro resistance to sodium stibogluconate using murine J774 macrophage amastigote testing method. The resistant isolates showed different restriction profiles when the amplified kDNA PCR products were digested with ALU1 restriction enzyme, indicating that resistance was mediated by different parasite clones
Description: 837-843
Keywords: Antimony Sodium Gluconate
Antiprotozoal Agents
Child, Preschool
DNA, Kinetoplast
DNA, Protozoan
Drug Administration Schedule
Drug Resistance
Endemic Diseases
Leishmaniasis, Visceral
Longitudinal Studies
Lymph Nodes
Parasitic Sensitivity Tests
Treatment Failure
Subject: Leishmania donovani
ISSN: 1020-3397
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Appears in Collections:EMRO Journal Articles (EMHJ)

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