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Title: Evaluation of 2-column agglutination versus conventional tube technique for antibody screening
Authors: Abou Jabal, A.
Shubeilat, T.
Hajjiri, F.
Issue Date: 2003
Language: English
Abstract: The study aimed to determine the specificity and sensitivity of the Ortho BioVue two-column agglutination system for the detection of low concentrations of clinically significant antibodies in serum. The BioVue system was compared with the conventional tube technique [LISS-Coombs indirect antiglobulin test], and the two-stage Papenzyme test was used to resolve discrepancies between the two methods. We tested 3000 serum samples from randomly selected patients at King Hussein Medical Centre. Both the antibody screening and identification gave negative results in 2952 patients and positive results in 48 patients. We found the BioVue system to be the more sensitive technique. However, if papain enzyme-treated cells were included in the conventional tube technique when applied to antibody screening and identification, both methods would be of comparable sensitivity
Description: 407-412
Keywords: Case-Control Studies
Cost-Benefit Analysis
Hemagglutination Tests
Isoantibodies
Mass Screening
Papain
Rho[D] Immune Globulin
Sensitivity and Specificity
Subject: Antibodies
URI: http://www.who.int/iris/handle/10665/119291
ISSN: 1020-3397
Other Identifiers: http://applications.emro.who.int/emhj/0903/emhj_2003_9_3_407_412.pdf
Appears in Collections:EMRO Journal Articles (EMHJ)



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