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Title: Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli
Authors: Jafari, A.
Katouli, M.
Shokouhi, F.
Bouzari, S.
Issue Date: 2001
Language: English
Abstract: The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates
Description: 756-762
Keywords: Bacterial Adhesion
Cell Culture Techniques
DNA Fingerprinting
DNA, Bacterial
Drug Resistance, Multiple, Bacterial
Escherichia coli O157
F Factor
Shiga Toxin 1
Shiga Toxin 2
Subject: Escherichia coli Infections
ISSN: 1020-3397
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Appears in Collections:EMRO Journal Articles (EMHJ)

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