Detection of Cryptosporidium parvum antigen by co-agglutination test and ELISA
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Abstract
Confirmation of the presence of Cryptosporidium in environmental samples is laborious, costly and often difficult. We report here a simple and economic slide agglutination test [co-agglutination test] for detecting cryptosporidial antigen in stool, serum and water. The results show that as a screening method co-agglutination is clearly superior to enzyme-linked immunosorbent assay [ELISA] and modified Ziehl-Neelsen staining, although ELISA is more accurate. The co-agglutination test is recommended for application as a new tool for detecting cryptosporidial antigen in large-scale epidemiological surveysCitation
(2000). Detection of Cryptosporidium parvum antigen by co-agglutination test and ELISA. http://www.who.int/iris/handle/10665/118944
Relation
EMHJ - Eastern Mediterranean Health Journal, 6 (5-6), 898-907, 2000
Description
898-907ISSN
1020-3397Other Identifiers
http://applications.emro.who.int/emhj/0605_6/EMHJ_2000_6_5-6_898_907.pdfCollections
Language
EnglishMetadata
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Intestinal parasites, including Cryptosporidium species, in Iraqi patients with sickle-cell anaemia
(2002)Stool samples were obtained from individuals admitted to three hospitals in Basra during November 1997-May 1998. Of 40 patients with sickle-cell anaemia, 25 [62.5%] had parasitic infections. In the apparently healthy comparison group, 26 of 175 individuals [14.8%] had intestinal parasitic infections, a statistically significant difference. The most common intestinal parasites isolated in the sickle-cell patients were Blastocystis hominis [36%] and Giardia lamblia [28%]. The isolation rate of Cryptosporidium species in sickle-cell patients [5%] ...
