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Title: Detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria by multiplex polymerase chain reactions
Authors: Mustafa, A.S.
Abal, A.T.
Chugh, T.D.
Issue Date: 1999
Language: English
Abstract: The ability of two-band and three-band multiplex polymerase chain reactions to detect and differentiate Mycobacterium tuberculosis complex from non-tuberculous mycobacteria was evaluated. The polymerase chain reactions differentiated between M. tuberculosis and non-tuberculous mycobacteria when standard strains and clinical isolates of mycobacteria were tested. The sensitivity of the two-band and three-band techniques to detect M. tuberculosis in clinical specimens, compared with smear and/or culture, was 88% and 75% respectively. Although both techniques showed 100% specificity, the superior sensitivity of the two-band technique suggests that it could be more useful in the diagnosis of tuberculosis and in differentiating M. tuberculosis complex from non-tuberculous mycobacteria
Description: 61-70
Keywords: DNA, Bacterial
Diagnosis, Differential
Mycobacterium
Mycobacterium Infections
Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity
Tuberculosis
Subject: Mycobacterium tuberculosis
URI: http://www.who.int/iris/handle/10665/118685
ISSN: 1020-3397
Other Identifiers: http://applications.emro.who.int/emhj/0501/EMHJ_1999_5_1_61_70.pdf
Appears in Collections:EMRO Journal Articles (EMHJ)



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