Files in This Item:


Title: Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodiumfaldparum and P. vivax
Authors: Nateghpour, M.
Abed Khojasteh, H.
Keshavarz, H.
Hajjaran, H.
Edrissian, Gh.
Rahimi, A.
Gobakhloo, N.
Issue Date: 2011
Language: English
Abstract: We compared light microscopy examination and a semi-nested multiplex PCR [SnM-PCR] assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small subunit ribosomal RNA [ssrRNA] gene sequences was applied. Microscopical examination showed that 48.5% [33.8% P. vivax and 14.7% P.falciparum] and 50% [35.3% P. vivax and 14.7% P.falciparum] of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy
Description: 51-55
Keywords: Malaria, Vivax
Polymerase Chain Reaction
Subject: Malaria, Falciparum
ISSN: 1020-3397
Other Identifiers:
Appears in Collections:EMRO Journal Articles (EMHJ)

Items in WHO IRIS are protected by copyright, with all rights reserved, unless otherwise indicated.