|Title:||Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodiumfaldparum and P. vivax|
Abed Khojasteh, H.
|Abstract:||We compared light microscopy examination and a semi-nested multiplex PCR [SnM-PCR] assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small subunit ribosomal RNA [ssrRNA] gene sequences was applied. Microscopical examination showed that 48.5% [33.8% P. vivax and 14.7% P.falciparum] and 50% [35.3% P. vivax and 14.7% P.falciparum] of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy|
Polymerase Chain Reaction
|Appears in Collections:||EMRO Journal Articles (EMHJ)|
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