Brucellosis is being reported with increasing frequency in the Islamic Republic of Iran. Serum antibodies in high-risk and general populations help to define cut-off levels and can be used as a simple and rapid diagnostic tests in infected areas. We performed the rose Bengal test [‏RBT]‏, serum agglutination test [‏SAT]‏ and 2-mercaptoethanol [‏2ME]‏ titre determination on 415 healthy individuals including butchers, slaughterers and others. Positive results were found by RBT, SAT titre [‏1:80]‏ and 2ME titre >/= 1:20 in slaughterers [‏10%, 20% and 6% ...